Our software package is suitable for cryo-electron microscopy of virus icosahedra, symmetry-mismatch, and local reconstruction. The package consists of three parts: icosprocess, icosprocess_mismatch, local_refine & local_reconstruct. Icosprocess (Icosahedral data process) is used to cryo-EM data process of icosahedral firstly, and it is extended to any symmetrical three-dimensional reconstruction now. The package includes detail manual, executable programs, and test dat. Icosprocess_mismatch is used to symmetry-mismatch reconstruction of cryo-EM based on the high-resolution 3D structure of icosahedral virus, and the orientation and center of each raw particle image. The package includes detail manual, executable programs, and test data. Local_refine & local_reconstruct are used to high-resolution map refinement. This software mainly uses local reconstruction and projection matching algorithm to refine the orientation and center of the local structure through iteration, and finally obtain the local structure of biological macromolecules with high resolution.

{"references": ["Xiaowu Li, Niyun Zhou, Wenyuan Chen, Bin Zhu, Xurong Wang, Bin Xu, Jiawei Wang, Hongrong Liu*, and Lingpeng Cheng*. Near-atomic resolution structure determination of a cypovirus capsid and polymerase complex using cryo-EM at 200 kV, J Mol Biol 2017, 429: 79-87.", "Hongrong Liu*,Lingpeng Cheng*,Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus, Science 2015,349: 1347-1350.", "Wenyuan Chen, Hao Xiao, Xurong Wang, Shuanglin Song, Zhen Han, Xiaowu Li, Fan Yang, Li Wang, Jingdong Song, Hongrong Liu, Lingpeng Cheng,Structural changes of a bacteriophage upon DNA packaging and maturation. Protein & Cell, 2020, 11, 374\u2013379."]}