Example dataset for bud-annotation plugin Napari. Here we provide two sets of 3 images of single molecule mRNA FISH on Saccheromyces cerevisiae (BY4741) strains containing an mRNA bud localization reporter at the DOA1 locus. The image set labeled EXPERIMENT contains images of the strain in which a localization element was present in the reporter mRNA. The reporter can be seen to localize to the bud. The image set labeled CONTROL contains images of the control strain without localization element present in the reporter mRNA. The reporter can be seen to be randomly distributed throughout the cell. Images were acquired as 41 z-stacks per fluorescence channel CY5, CY3.5, CY3 and DAPI. For each fluorescence image a corresponding DIC image was acquired as well. The following smFISH probes were used: - CY5: probes targeting the endogenous ASH1 and CLB2 mRNA to be used as bud marker (Quasar 670) - CY3.5: probes targeting the DOA1 mRNA reporter (CAL Fluor Red 610) - CY3: probes targeting the MS2v6 sequence inserted at the 3' end of the DOA1 mRNA reporter (Quasar 570) FISH-QUANT spot analysis results for the CY3.5 channel from these images are included. This data can be used to extract the spot information and assign spots to either mother cell or bud. Examples of nuclear, bud and cell masks for all images are provided as well.