Dataset of kinetochore tracks in human RPE1 cells showing chromosome dynamics and segregation from prometaphase through to anaphase as described in detail in Sen, Harrison, Burroughs and McAinsh, 2021, https://doi.org/10.1101/2021.03.30.436326 Tracks correspond to 3D time-lapse movies of Ndc80-eGFP and were acquired in the 488nm channel using 1\% laser power, 50 ms exposure time/z-plane, 93 z-planes, 307 nm z-step, which results in 4.7 s/z-stack time frame. Cells are subject to nocodazole arrest-and-release or equivalent treatment with DMSO as indicated in the folder names, and some cells are subject to additional treatment with ZM to inhibit Aurora B (also indicated in folder names). Tracks were produced using kinetochore tracking software, KiT v2.3 (see Armond et al., 2016, Bioinformatics), available from https://github.com/cmcb-warwick/KiT/

{"references": ["Sen, Harrison et al, 2021, https://doi.org/10.1101/2021.03.30.436326"]}