Surveys in the southern desert-environment of Tunisia, particularly in the Jbil National Park and its neighbouring areas showed important animal diversity despite the drought and harsh conditions characterizing those regions. Investigation during recent years has concerned three main groups widely known, namely mammals, birds and reptiles, totalling at least 15, 60 and 10 species in the region respectively. In this paper we are principally interested in the Slender-horned gazelle, Dorcas gazelle, and Houbara bustard considered emblematic species of the desert zone which have potential to contribute to interest in ecotourism. The distribution, habitat requirements and the main threats of these species were given special attention in this paper in order to contribute in the biodiversity conservation.