This data set accompanies the publication "Dynamic modelling of phosphorolytic cleavage catalyzed by pyrimidine-nucleoside phosphorylase" in MDPI Processes, and is a collection of UV/vis spectra used for monitoring of reaction progress under various experimental conditions. The monitored reaction is thymidine phosphorolysis, catalyzed by an enzyme (EC 2.4.2.2). Phosphate and thymidine concentrations are in the range of 2–80 mM and 0.8–5 mM, respectively, and final enzyme concentration in the range of 12.5–50 µg/mL. The recorded data represents time courses over 24 hours for 48 reaction conditions. This collection also contains the documentation of all work done in the laboratory to achieve this data (provenance in the form of the complete lab journal).

Funding for this research was received from: - German Research Foundation (DFG) under Germany´s Excellence Strategy – EXC 2008/1– 390540038 (UniSysCat) - Einstein Foundation Berlin (ESB) - Einstein Center of Catalysis (EC²)