This was the second mobilisation of MARCO-BOLO’s Community of Practice (CoP). The purpose of this 2nd CoP Workshop was to: Advance the process of stakeholder consultation for the creation of MARCO-BOLO products as key solutions to the challenges currently facing marine biodiversity, ensuring that the needs and requirements of end-users are met. The project’s Data Management Plan and new technology based on tools such as eDNA, artificial intelligence and 3D image analysis, were addressed in detail. The subsequent discussions included input from stakeholders to guide further development. The meeting counted on the collaboration of the OBAMA-NEXT project as a relevant sister initiative. This second Co-Design/Co-Creation Workshop was part of the event “The Future of Marine Biodiversity Monitoring in Europe” organised by the ACTeon research consultancy for the Joint Research Center.