This methodological deliverable presents the Cost-Benefit Analysis (CBA) framework developed within the PathOS project to assess the impacts of Open Science initiatives. Drawing on established CBA principles and refined through real-world applications to UniProt and RCAAP, the framework offers a structured, rigorous approach to quantify both the costs and benefits of Open Science practices. It enables comparisons with a non-Open Science scenario, identifies typical cost-benefit categories, and provides guidance for their measurement. The framework focuses on short-term, measurable outcomes while acknowledging potential long-term enablement effects. A glossary and technical annex support users in applying the method. This is an updated version of the previous version of the D4.1 Methodological note on the CBA of Open Science practices.

This project deliverable is currently pending approval by the EC.