Small wedge MicroED data of Lysozyme, low-dose MTH1-8DG and high-dose MTH1-8DG. A. Data information Dataset 1: Lysozyme Dataset 2: MTH1 with ligand 8-OXO-2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE (PDB code: 8DG) collected using high fluence. Dataset 3: MTH1 with ligand 8-OXO-2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE (PDB code: 8DG) collected using low fluence. B. Data collection Data was collected with a CMOS CetaD detector installed on a Titan Krios G2 with an autoloader. Data collection software: EPUD Cryo-EM infrastructure unit, Scilifelab, Stockholm. C. Instrument and measurement settings Instrumental: Instrument Titan Krios G3i Radiation source FEG Accelerating voltage 300 kV Wavelength 0.01968 Å Probe Type Nanoprobe Beam Diameter 1.5 μm Beam Convergence Parallel beam Detector Ceta-D CMOS detector Number of pixels in the image 1024 x 1024 Pixel size 5.6 µm x 5.6 µm Effective Camera Length 3019.12 (lowdose), 3019.12 (highdose) and 1166 (lysozyme) Hardware Binning 4 Experimental: Data Type Electron diffraction data - 3D ED, Microcrystal diffraction (MicroED) Data collection method Continuous rotation Temperature (K) used during data collection 77 K Number of crystals contributing to the data set 25 (lowdose), 20 (highdose), 61 (lysozyme) Number of experimental frames 20 frame/data (lowdose), 20 frame/data (highdose), 30 frame/data (lysozyme) tilt range, exposure time, tilt per frame lowdose: 20 degree, 1s, 1 degree/frame highdose: 20 degree, 1s, 1 degree/frame lysozyme: 15 degree, 1s, 0.5 degree/frame Software: Software used for the data collection EPUD Software used for processing AutoLEI with XDS Authorship and Bibliography Author(s) of the data Lei Wang (ESR 10) Related data Publication(s) Files and data formats Image format MRC