The benchmark dataset of the 2nd AnDi Challenge has been developed to test and compare methods for analyzing diffusion trajectories with diffusive changes. It explores biological and physical scenarios such as multistate diffusion, trapping, confinement, and dimerization. Each experiment targets specific characteristics—ranging from broad diffusive parameters to extreme trapping conditions—offering a comprehensive foundation for evaluating changepoint detection, classification, and characterization methods. For further details and documentation, see the associated content: Website of the project: http://andi-challenge.org/ Website of the Challenge: https://codalab.lisn.upsaclay.fr/competitions/16618 Tutorial explaining how this dataset has been generated: https://github.com/AnDiChallenge/andi_datasets/blob/master/source_nbs/tutorials/challenge_two_benchmark.ipynb Preprint explaining all details of the 2n AnDi Challenge: https://arxiv.org/abs/2311.18100