ABSTRACT Some elements play a highly important role in our human body. We require these elements to keep our human body healthy and active. If the required elements are not taken in sufficient amount, it can cause symptoms of nutritional deficiency and many other disorders. However, our needs for these elements are easily met by eating a variety of foods from the different food groups. Our research was based on two important elements i.e. Magnesium (Mg) and Calcium (Ca)). We studied their importance in the human body as well as the effect they have on human health and behaviour when their maintained concentrations in the blood are misbalanced. For this purpose a group of volunteers were chosen from two geologically diverse environments, one being a natural and village environment called Hunza,located in the mountainous region of Pakistan and the other, Karachi, which is a busy and heavily polluted city located in the heart of Pakistan, which is a third world country. The concentrations of these two elements in their blood serum was measured using Flame Atomic Absorption Spectroscopy and were compared with the data provided by the volunteers related to their symptoms and behaviour. As the world moves towards modernization, the decline in the health of young individuals is increasing alarmingly with the decline in the consumption of natural products and environmental pollutants. This causes medical problems like weakness, hair loss, arrhythmia and many more like these to be prevalent in today’s generation at an alarming rate. Furthermore, behavioural changes like anxiety, anger, and depression are also found very commonly in today’s generation. This study aims at determining the relationship between these two essential elements and how their increased and decreased concentration in human blood serum affects all these factors. It also includes a detailed statistical analysis done using SPSS software for calculating the Mean, Median and the standard deviation for both the rural and urban region.. A connection was observed between people suffering from low levels of Calcium and Magnesium in their blood serum and disorders like hair loss, insomnia, muscle fatigue, joint pain and hyper activity. Behavioural symptoms like anger, frustration and anxiety was also linked with magnesium and calcium deficiency