SGC Open Notebook Project to Characterize the HMTase NSD3 Exp021 Objective: During epithelial to mesenchymal transitioning (EMT), transcription networks become activated and cytoskeletal rearrangements take place. These events promote migratory capacity and invasiveness. Cadherins are a family of cell adhesion molecules central in transitioning to a more mesenchymal state. E-cadherin, a marker for polarized epithelial cells, is downregulated during EMT. Here, I investigate the effect of NSD3short overexpression on E-cadherin expression in H1299 cells, which would support NSD3 as a EMT driver.

Funding Acknowledgment: The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.