This document provides measurement protocols for dimensional properties, accuracy determination and measurements of microfluidic components. The measurement protocols serve as a guide for NMI/DI planning to introduce new calibration services, such as the internal dimensions of channels (diameter/length/width). Finally, the protocol includes an example where the glass transfer standards produced in A2.4.2 have been tested according to this document. Activity number Activity description Partners (Lead in bold) A3.3.3 M33 Using input from A4.3.1 and A4.3.2, RISE with support from IMTAG, microfluidic, BHT and LNE will define by April 2023 (M23) measurement protocols for dimension characteristics, accuracy determination and measurements of microfluidic components. The measurement protocols will be used for building the transfer standards in A2.4.2. The protocols will be used also for testing the transfer standards once they are built, and this testing will in turn lead to updating the protocols by February 2024 (M33). Once agreed by the consortium, the coordinator on behalf of RISE, IMTAG, microfluidic, BHT and LNE will submit the measurement protocols to EURAMET as D8: ‘Measurement protocols for dimensional characterisation of microfluidic components’. These measurement protocols will be used as guidelines for NMI/DIs wishing to introduce new calibration services such as inner dimensions of channels (diameter/length/width). RISE, IMTAG, LNE, microfluidic, BHT