In December 2022, 196 Parties to the CBD adopted the Global Biodiversity Framework (GBF) and with it the Ne 500 and Populations Maintained indicators as headline and complementary indicators, respectively, in the accompanying global monitoring framework (Annex 1 of CBD/COP/DEC/15/5). The following guideline materials are intended to assist nations in quantifying genetic indicator values at every stage of the process: from species selection to data compilation to indicator calculation. The materials are based on the co-creation experience of the first pilot multinational assessment of the genetic diversity indicators, and will be regularly kept updated through a versioning system as more teams share their experience. The first release was submitted to Biodiversity Informatics as "Training module" and presented at the 16 April 2024 CBD Webinar on the guidance from the AHTEG on indicatros for the GBF.