
This report presents a guide to selecting high-quality commercial antibodies against FIG4 (Polyphosphoinositide phosphatase)by Western blot, immunoprecipitation and immunofluorescence, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. This study was funded in part by the ALS-Reproducible Antibody Platform (ALS-RAP), established as a public-private partnership by three prominent ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.
Polyphosphoinositide phosphatase, antibody characterization, SAC domain-containing protein 3, Western blot, Q92562, FIG4, Serine-protein phosphatase FIG4, immunofluorescence, antibody validation, Phosphatidylinositol 3,5-bisphosphate 5-phosphatase, immunoprecipitation
Polyphosphoinositide phosphatase, antibody characterization, SAC domain-containing protein 3, Western blot, Q92562, FIG4, Serine-protein phosphatase FIG4, immunofluorescence, antibody validation, Phosphatidylinositol 3,5-bisphosphate 5-phosphatase, immunoprecipitation
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