Ascorbic acid is required by both plants and animals for the essential metabolic process. It is sufficiently available in the citrus fruits, tomatoes, potatoes, red peppers, milk, oysters, animal liver, etc. In this study, the LC-MS and GC-MS were used to characterize the structural properties and isotopic abundance ratio to evaluate the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on L-ascorbic acid compared to the control sample. The test sample was divided into two parts, one part of ascorbic acid was considered as a control sample, while the second part only received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi and termed as a Biofield Energy Treated sample. The LC-MS spectra of both the control and Treated samples at retention time (Rt) 1.8minutes exhibited the mass of the deprotonated molecular ion peak at m/z 175 [M-H]- (calculated for C6H7O6-, 175.02). The peak area of the treated sample (3607446.27) was significantly decreased by 65.05% compared to the control sample (10320794.8). The LC-MS based isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in the treated ascorbic acid was significantly increased by 119.71% compared with the control sample. Thus, 13C, 2H, and 17O contributions from (C6H7O6)- to m/z 176 in the treated sample were significantly increased compared with the control sample. The gas chromatographic peak area of the treated sample (12.74) was significantly decreased by 31.1% compared to the control sample (18.49). Similarly, the GC-MS based isotopic abundance ratio of PM+2/PM in the treated ascorbic acid was significantly increased by 28.74% compared with the control sample. So, 18O contributions from (C6H8O6)+ to m/z 177 in the Biofield Energy Treated sample were significantly increased compared with the control sample. The isotopic abundance ratio of PM+1/PM (18O/16O) in the treated ascorbic acid was significantly increased compared to the control sample. The increased isotopic composition of the Trivedi Effect®-Consciousness Energy Healing Treated ascorbic acid might have altered the neutron to proton ratio in the nucleus. The changes in isotopic abundance could be due to changes in nuclei possibly through the interference of neutrino particles via the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio and peak area of the treated ascorbic acid may increase the intra-atomic bond strength, increase its stability. This new form of ascorbic acid would be very useful to design the better pharmaceutical formulations that might offer better therapeutic response against scurvy, obesity, cardiovascular diseases, hypertension, Alzheimer's disease, cancer, rheumatoid arthritis, etc. Journal Web URL:- https://crimsonpublishers.com/acam/fulltext/ACAM.000638.php