ABSTRACT The emergence as well as resistance of pathogenic microorganisms currently poses a particularly serious public health problem. The use of medicinal plants is therefore one of the most interesting avenues to explore. It is with this in mind that this work was carried out and aims to evaluate the activity of citrus sinensis bark on the growth of Candida albicans and Trichophyton rubrum. In this study, we proceeded to the hydroethanolic extraction at 70% of the bark of this plant and the extract was tested by the method of double dilution. The results showed that the two microbial strains are sensitive to the ethanolic extract of Citrus sinensis according to the dose-response relationship with an MIC value of 50mg/mL for Candida albicans and an MIC value greater than 50mg/mL for Trichophyton rubrum. The extract is fungistatic against Candida albicans but has weak inhibitory activity for Trichophyton rubrum. Keywords: Antifungal activity- Citrus sinensis - mycosis