research data . Dataset . 2016

Convergent Transmission of RNAi Guide-Target Mismatch Information across Argonaute Internal Allosteric Network

T. Joseph, Thomas; Osman, Roman;
Open Access
  • Published: 01 Jan 2016
  • Publisher: Figshare
Abstract
<div><p>In RNA interference, a guide strand derived from a short dsRNA such as a microRNA (miRNA) is loaded into Argonaute, the central protein in the RNA Induced Silencing Complex (RISC) that silences messenger RNAs on a sequence-specific basis. The positions of any mismatched base pairs in an miRNA determine which Argonaute subtype is used. Subsequently, the Argonaute-guide complex binds and silences complementary target mRNAs; certain Argonautes cleave the target. Mismatches between guide strand and the target mRNA decrease cleavage efficiency. Thus, loading and silencing both require that signals about the presence of a mismatched base pair are communicated ...
Subjects
free text keywords: Biophysics, Chemistry, convergent, rnai, guide-target, mismatch, argonaute, allosteric
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Dataset . 2016
Provider: figshare
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