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[Effects of fibroblasts on the proliferation and migratory capacity of renal cell carcinoma cell lines and sensitivity to everolimus].
Copyright policy )[Effects of fibroblasts on the proliferation and migratory capacity of renal cell carcinoma cell lines and sensitivity to everolimus].
To observe the effects of normal human renal fibroblast (NHRF) on renal cell carcinoma cell lines.Renal cell carcinoma (RCC) cell lines 786-O and Caki-1 were co-cultured with NHRF for assessing the proliferation and migratory capacities of renal cell carcinoma cell lines and the sensitivity to everolimus.Co-culturing with NHRF substantially increased the proliferation capacity of both RCC cell lines (786-O: 2.35 ± 0.05 vs1.93 ± 0.15, P = 0.01;Caki-1: 2.35 ± 0.21 vs 1.24 ± 0.11, P = 0.001). Similarly the migratory capacities of both cell lines became significantly enhanced after co-culturing (786-O: 1.53 ± 0.11 vs 0.98 ± 0.11, P = 0.04; Caki-1: 1.53 ± 0.11vs 0.98 ± 0.11, P = 0.04) compared with untreated control. Furthermore, the sensitivities of both cell lines to everolimus (1, 5, 10 µmol/L) dramatically decreased in those pre-co-cultured with NHRF (786-O:P value 0.04, 0.35, 0.18); Caki-1: P value 0.02, 0.03, 0.024).NHRF promotes the proliferation and migration capacities of 786-O and Caki-1. And it may be involved in the resistance of RCC to everolimus.
Carcinoma, Renal Cell, Cell Line, Tumor, Cell Movement, Cell Proliferation, Everolimus, Fibroblasts, Humans, Kidney, Kidney Neoplasms, Sirolimus
Carcinoma, Renal Cell, Cell Line, Tumor, Cell Movement, Cell Proliferation, Everolimus, Fibroblasts, Humans, Kidney, Kidney Neoplasms, Sirolimus
To observe the effects of normal human renal fibroblast (NHRF) on renal cell carcinoma cell lines.Renal cell carcinoma (RCC) cell lines 786-O and Caki-1 were co-cultured with NHRF for assessing the proliferation and migratory capacities of renal cell carcinoma cell lines and the sensitivity to everolimus.Co-culturing with NHRF substantially increased the proliferation capacity of both RCC cell lines (786-O: 2.35 ± 0.05 vs1.93 ± 0.15, P = 0.01;Caki-1: 2.35 ± 0.21 vs 1.24 ± 0.11, P = 0.001). Similarly the migratory capacities of both cell lines became significantly enhanced after co-culturing (786-O: 1.53 ± 0.11 vs 0.98 ± 0.11, P = 0.04; Caki-1: 1.53 ± 0.11vs 0.98 ± 0.11, P = 0.04) compared with untreated control. Furthermore, the sensitivities of both cell lines to everolimus (1, 5, 10 µmol/L) dramatically decreased in those pre-co-cultured with NHRF (786-O:P value 0.04, 0.35, 0.18); Caki-1: P value 0.02, 0.03, 0.024).NHRF promotes the proliferation and migration capacities of 786-O and Caki-1. And it may be involved in the resistance of RCC to everolimus.