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Article . 1992
Science
Article . 1992 . Peer-reviewed
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constructing proteins by dovetailing unprotected synthetic peptides backbone engineered hiv protease

Authors: Martina Schnölzer; Stephen B. H. Kent;

constructing proteins by dovetailing unprotected synthetic peptides backbone engineered hiv protease

Abstract

Backbone-engineered HIV-1 protease was prepared by a total chemical synthesis approach that combines the act of joining two peptides with the generation of an analog structure. Unprotected synthetic peptide segments corresponding to the two halves of the HIV-1 protease monomer polypeptide chain were joined cleanly and in high yield through unique mutually reactive functional groups, one on each segment. Ligation was performed in 6 molar guanidine hydrochloride, thus circumventing limited solubility of protected peptide segments, the principal problem of the classical approach to the chemical synthesis of proteins. The resulting fully active HIV-1 protease analog contained a thioester replacement for the natural peptide bond between Gly 51 -Gly 52 in each of the two active site flaps, a region known to be highly sensitive to mutational changes of amino acid side chains.

Related Organizations
Keywords

Models, Molecular, Protein Conformation, Molecular Sequence Data, Guanidines, Mass Spectrometry, HIV Protease, HIV-1, Indicators and Reagents, Amino Acid Sequence, Peptides, Guanidine

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    334
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Found an issue? Give us feedback
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
334
Top 10%
Top 1%
Top 1%
bronze