To investigate the expression of IGF-IR and PKC and their clinical significance in laryngeal squamous cell carcinoma(LSCC).The expression of IGF-IR and PKC protein were detected by immunohistochemistry and Western blot in 60 cases of LSCC, 25 cases of adjacent non-tumorous laryngeal epithelium and 10 cases of normal laryngeal epithelium.The positive rate of IGF-IR and PKC expression in LSCC were significantly higher than that in normal laryngeal epithelium and adjacent non-tumorous laryngeal epithelium (P < 0.05). IGF-IR positive expression were increased more in cases with stage III-IV, cervical lymph node metastasis and well differentiation than that in cases with stage I-II, non-lymph node metastasis and poor/mediate differentiation, respectively (P < 0.05). The positive rate of PKC in LSCC were correlated with clinical stage and cervical lymph node metastasis. There was a correlation between the expression of IGF-IR and PKC in LSCC.The overexpression of IGF-IR and PKC in the laryngeal carcinoma may play an important role in the carcinogenesis and development of LSCC. It is suggested that detecting the expression of IGF-IR and PKC can be used for early diagnosis and treatment of LSCC.