publication . Article . 2009

Adhesion and growth inhibitory effect of chicken egg yolk antibody (IgY) on Salmonella enterica serovars Enteritidis and Typhimurium in vitro.

Chalghoumi, Raja; Thewis, André; Beckers, Yves; Marcq, Christopher; Portetelle, Daniel; Schneider, Yves-Jacques;
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  • Published: 01 Jan 2009 Journal: Foodborne Pathogens and Disease, volume 6, issue 5, page 593-604
  • Country: Belgium
Abstract
The protective effects of powder preparation of egg yolk immunoglobulin Y (IgY), specific to Salmonella Enteritidis and Salmonella Typhimurium outer membrane proteins (OMP), against these two Salmonella sp. serovars were investigated in vitro in two different assays: adhesion-prevention and growth-inhibition. The adhesion-prevention assay was conducted using polarized monolayers of the human intestinal epithelial Caco-2 cell line. First, the conditions of Salmonella adherence to Caco-2 cells were optimized, and interferences of bacteria with the transepithelial electrical resistance (TER) of fully differentiated Caco-2 cell monolayers and the lactate dehydrogena...
Subjects
free text keywords: Immunoglobulin Y, Salmonella spp., in vitro adhesion, Caco-2 cells, in vitro growth, Life sciences, Veterinary medicine & animal health, Animal production & animal husbandry, Microbiology, Sciences du vivant, Médecine vétérinaire & santé animale, Productions animales & zootechnie, Microbiologie
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