publication . Article . 2009

High-Throughput Screening Techniques for Biomass Conversion

Roman Brunecky;
  • Published: 14 Oct 2009 Journal: BioEnergy Research, volume 2, pages 179-192 (issn: 1939-1234, eissn: 1939-1242, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
High-throughput (HTP) screening of biomass or biomass-degrading enzymes, regardless of the desired outcome, is fraught with obstacles and challenges not typically faced in more traditional biotechnology. The enzyme systems are complex and synergistic and the substrate is highly heterogeneous, insoluble, and difficult to dispense. Digestions are often carried out for days at temperatures of 50°C or higher, leading to significant challenges regarding evaporation control in small well volumes. Furthermore, it is often desirable to condition or “pretreat” the biomass at extreme temperatures and/or pH to enhance enzyme digestibility. Once the substrate has been sacch...
free text keywords: Agronomy and Crop Science, Renewable Energy, Sustainability and the Environment, Energy (miscellaneous)
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