publication . Article . 2012

hptlc and reverse phase hplc methods for the simultaneous quantification and in vitro screening of antioxidant potential of isolated sesquiterpenoids from the rhizomes of cyperus rotundus

M. Priya Rani; K.P. Padmakumari;
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  • Published: 27 Jun 2012 Journal: Journal of Chromatography B, volume 904, pages 22-28 (issn: 1570-0232, Copyright policy)
  • Publisher: Elsevier BV
Abstract
Abstract Three sesquiterpenoids solavetivone, aristolone and nootkatone were isolated from the acetone extract of Cyperus rotundus by silica gel column chromatography and identified by spectral studies. Solavetivone has been isolated for the first time from the species. Simple, sensitive and selective HPTLC and HPLC methods with ultraviolet detection (245 nm) were developed and validated for the simultaneous quantification. HPTLC method was validated in terms of their linearity, LOD, LOQ, precision, accuracy and compared with RP-HPLC-UV method. Among the three sesquiterpenoids isolated, nootkatone possessed the highest radical scavenging potential (IC50 4.81 μg/...
Subjects
free text keywords: Analytical Chemistry, Clinical Biochemistry, Cell Biology, Biochemistry, General Medicine, High-performance liquid chromatography, Reversed-phase chromatography, Silica gel, chemistry.chemical_compound, chemistry, Column chromatography, Cyperus rotundus, Nootkatone, DPPH, Antioxidant, medicine.medical_treatment, medicine, Chromatography
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