publication . Article . 1996

Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor.

Taekjip Ha; Paul R. Selvin; Shimon Weiss; Th Enderle; Daniel S. Chemla; Daniel S. Chemla; D. F. Ogletree;
Open Access
  • Published: 25 Jun 1996 Journal: Proceedings of the National Academy of Sciences, volume 93, pages 6,264-6,268 (issn: 0027-8424, eissn: 1091-6490, Copyright policy)
  • Publisher: Proceedings of the National Academy of Sciences
Abstract
Abstract We extend the sensitivity of fluorescence resonance energy transfer (FRET) to the single molecule level by measuring energy transfer between a single donor fluorophore and a single acceptor fluorophore. Near-field scanning optical microscopy (NSOM) is used to obtain simultaneous dual color images and emission spectra from donor and acceptor fluorophores linked by a short DNA molecule. Photodestruction dynamics of the donor or acceptor are used to determine the presence and efficiency of energy transfer. The classical equations used to measure energy transfer on ensembles of fluorophores are modified for single-molecule measurements. In contrast to ensem...
Subjects
arXiv: Quantitative Biology::BiomoleculesPhysics::Biological PhysicsQuantitative Biology::Subcellular ProcessesPhysics::Chemical Physics
free text keywords: Macromolecule, Single-molecule FRET, Molecular physics, Analytical chemistry, Fluorophore, chemistry.chemical_compound, chemistry, Near-field scanning optical microscope, Förster resonance energy transfer, Acceptor, Emission spectrum, Molecule
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