publication . Article . 2007

Transcytosis of gastrointestinal epithelial cells by Escherichia coli K1.

Jane L Burns; Adam Griffith; John J Barry; Mechthild Jonas; Emil Y Chi;
Open Access
  • Published: 13 Apr 2007 Journal: Pediatric Research, volume 49, pages 30-37 (issn: 0031-3998, eissn: 1530-0447, Copyright policy)
  • Publisher: Springer Nature
Abstract
Escherichia coli K1 is an important neonatal pathogen that is usually transferred from maternal to infant gastrointestinal tract at the time of parturition. Approximately 20% of neonates are colonized, and a proportion of colonized infants goes on to have systemic infection. Entry into the bloodstream from the gastrointestinal tract is hypothesized to occur via epithelial cell invasion. Invasion of multiple epithelial cell lines was studied using gentamicin protection assays and transcytosis of polarized monolayers. Electron microscopy was used to confirm cellular invasion. Cell lines used include two human gastrointestinal lines, Caco-2 and T84; a human respira...
Subjects
Medical Subject Headings: digestive system
free text keywords: Biology, Tissue culture, Cell culture, Enterobacteriaceae, biology.organism_classification, Epithelium, medicine.anatomical_structure, medicine, Transcytosis, Microbiology, Escherichia coli, medicine.disease_cause, Gastrointestinal tract, In vitro
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