publication . Article . 2002

Gene regulation by low level UV-B radiation: identification by DNA array analysis.

Åke Strid;
  • Published: 11 Sep 2002 Journal: Photochem. Photobiol. Sci., volume 1, pages 656-664 (issn: 1474-905X, eissn: 1474-9092, Copyright policy)
  • Publisher: Royal Society of Chemistry (RSC)
UV-B radiation alters transcript levels of various defence genes and photosynthetic genes in plants. Utilising a DNA array with 5000 ESTs and cDNAs from Arabidopsis thaliana, 70 genes were found to show a greater than two-fold induction or repression of transcript levels. Six genes (MEB5.2, PyroA, Ubq3, Lhcb6, F5D21.10 and the gene for an RNA polymerase II subunit) were tested for stress specific gene regulation on northern blots with RNA from plants exposed to low dose UV-B radiation, ozone or wounding. Transcript levels for PyroA, Ubq3 and the gene for a RNA polymerase II subunit were all specifically increased by UV-B. MEB5.2 mRNA levels also rose, whereas Lh...
free text keywords: Physical and Theoretical Chemistry
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