publication . Article . 2003

Long Distance Communication between Muscarinic Receptors and Ca2+ Release Channels Revealed by Carbachol Uncaging in Cell-attached Patch Pipette

Alexei Tepikin; Oleg Gerasimenko; Cristina Camello;
Open Access
  • Published: 25 Mar 2003 Journal: Journal of Biological Chemistry, volume 278, pages 20,860-20,864 (issn: 0021-9258, eissn: 1083-351X, Copyright policy)
  • Publisher: American Society for Biochemistry & Molecular Biology (ASBMB)
We have investigated the characteristics of cytosolic Ca2+ signals induced by muscarinic receptor activation of pancreatic acinar cells that reside within intact pancreatic tissue. We show that these cells exhibit global Ca2+ waves and local apical Ca2+ spikes. This is the first evidence for local Ca2+ signaling in undissociated pancreatic tissue. The mechanism of formation of localized Ca2+ signals was examined using a novel approach involving photolysis of caged carbachol inside a patch pipette attached to the basal surface of an acinar unit. This local activation of basal muscarinic receptors elicited local cytosolic Ca2+ spikes in the apical pole more than 1...
free text keywords: Cell Biology, Biochemistry, Molecular Biology
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