publication . Article . 2007

dna from formalin fixed tissue extraction or repair that is the question

Morten Skage; Christoffer Schander;
  • Published: 16 Nov 2007 Journal: Marine Biology Research, volume 3, pages 289-295 (issn: 1745-1000, eissn: 1745-1019, Copyright policy)
  • Publisher: Informa UK Limited
Abstract Establishing effective DNA-based protocols for use on archival material fixed in formaldehyde (formalin) is a particularly challenging task. Formalin fixation induces cross-linking with nucleic acids and proteins, thereby reducing the amount and quality of the extracted DNA. Previous attempts have primarily focused on optimizing DNA extraction protocols. Here we focus on the use of enzymes capable of in vitro repair of DNA extracts prior to amplification of the nucleic acids by the polymerase chain reaction (PCR). The amplification success of mitochondrial DNA was greater using the repair enzyme assay (56%) than with the regular PCR assay (20%), and eve...
free text keywords: Aquatic Science, Ecology, Evolution, Behavior and Systematics, Oceanography, Real-time polymerase chain reaction, DNA damage, DNA repair, DNA extraction, DNA, chemistry.chemical_compound, chemistry, Ligase chain reaction, Molecular biology, Polymerase chain reaction, law.invention, law, Multiple displacement amplification, Biology
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