publication . Article . 2015

a novel in vitro assay for assessing efficacy and toxicity of antifungals using human leukaemic cells infected with candida albicans

J. Boros-Majewska; Ł. Turczyk; X. Wei; S. Milewski; D.W. Williams;
  • Published: 01 May 2015 Journal: Journal of Applied Microbiology, volume 119, pages 177-187 (issn: 1364-5072, Copyright policy)
  • Publisher: Wiley
Aims This study describes a novel in vitro assay that simultaneously determines antifungal efficiency and host cell toxicity using suspensions of human leukemic cells (HL-60) infected with Candida albicans. Methods and Results The effect of Candida infection on host cell viability was evaluated by microscopy of trypan blue stained cells and lactate dehydrogenase (LDH) activity. The in vitro ‘drug potency assay’ utilized the Cell Counting Kit-8 and measured post antifungal treatment viability of Candida-infected HL-60 cells and ability of the antifungal to prevent infection. LDH activity showed that 42% ± 4.0 and 85.3% ± 7.40 of HL-60 cells were killed following ...
free text keywords: Nystatin, medicine.drug, medicine, Propidium iodide, chemistry.chemical_compound, chemistry, Biology, Multiplicity of infection, Microbiology, Candida albicans, biology.organism_classification, Lactate dehydrogenase, Trypan blue, Cytotoxicity, Corpus albicans
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