publication . Article . 2000

Molecular identification of Acetobacter isolates from submerged vinegar production, sequence analysis of plasmid pJK2-1 and application in the development of a cloning vector.

J. Trček; P. Raspor; M. Teuber;
  • Published: 13 Mar 2000 Journal: Applied Microbiology and Biotechnology, volume 53, pages 289-295 (issn: 0175-7598, eissn: 1432-0614, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
Three new Acetobacter strains were isolated from vinegar. By plasmid profiling they were recognized as genotypically different from each other. Sequencing of the genes for 16S and 23S rRNA and DNA–DNA hybridization of total DNA against DNA of all type strains of Acetobacter identified Acetobacter strains JK2 and V3 as A. europaeus, and Acetobacter strain JK3 as A. intermedius. In contrast to the type strain of A. europaeus (DSM 6160), A. europaeus JK2 and V3 do not require acetic acid for growth and can be successfully transferred between media with and without acetic acid. This phenotypic characteristic enables convenient handling of both strains in genetic stu...
free text keywords: Biotechnology, Applied Microbiology and Biotechnology, General Medicine
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