publication . Article . 2008

a new quantitative rt pcr method for sensitive detection of dengue virus in serum samples

Nadine Sadon; Anne Delers; Richard G. Jarman; Chonticha Klungthong; Ananda Nisalak; Robert V. Gibbons; Ventzislav Vassilev;
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  • Published: 13 Aug 2008 Journal: Journal of Virological Methods, volume 153, pages 1-6 (issn: 0166-0934, Copyright policy)
  • Publisher: Elsevier BV
Abstract
In order to detect and identify dengue serotypes in serum samples, we developed a single-step quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay (referred to as Q-PCR). Sets of primers were selected from the capsid region of the viral genome. Dengue serotypes 1/3 and 2/4 were detected in two separate duplex amplification reactions using specific primers and fluorogenic TaqMan™ probes. Results obtained with this Q-PCR and the classical nested RT-PCR (N-PCR) assays were compared using a panel of 97 representative human sera collected from patients in Bangkok, Thailand. It is shown that the Q-PCR is a rapid, sensitive and reproducible too...
Subjects
free text keywords: Virology, Dengue fever, medicine.disease, medicine, Real-time polymerase chain reaction, Flavivirus, biology.organism_classification, biology, Serotype, Virus, Molecular biology, Dengue virus, medicine.disease_cause, Reverse transcription polymerase chain reaction, Flaviviridae
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