publication . Article . 2002

osteoblast mediated mineral deposition in culture is dependent on surface microtopography

B.D. Boyan; L.F. Bonewald; E.P. Paschalis; C.H. Lohmann; J. Rosser; D.L. Cochran; D.D. Dean; Z. Schwartz; A.L. Boskey;
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  • Published: 01 Dec 2002 Journal: Calcified Tissue International, volume 71, pages 519-529 (issn: 0171-967X, eissn: 1432-0827, Copyright policy)
  • Publisher: Springer Science and Business Media LLC
Abstract
Osteoblast phenotypic expression in mono- layer culture depends on surface microtopography. Here we tested the hypothesis that mineralized bone nodule formation in response to osteotropic agents such as bone morphogenetic protein-2(BMP-2 ) and dexameth- asone is also influenced by surface microtopography. Fetal rat calvarial (FRC) cells were cultured on Ti im- plant materials (PT (pretreated), Ra = 0.6 lm; SLA (course grit blasted and acid etched), Ra = 4.0 lm; TPS (Ti plasma sprayed), Ra = 5.2 lm) in the presence of either BMP-2(2 0 ng/ml) or 10 )8 M dexamethasone (Dex). At 14 days post-confluence, a homogenous layer of cells covered the surfaces, and stacks of...
Subjects
free text keywords: Endocrinology, Diabetes and Metabolism, Endocrinology, Orthopedics and Sports Medicine, Osteoblast, medicine.anatomical_structure, medicine, Alkaline phosphatase, Metallurgy, Cell growth, Growth factor, medicine.medical_treatment, Molecular biology, Bone cell, Apatite, visual_art.visual_art_medium, visual_art, Bone morphogenetic protein 2, Mineralization (biology), Internal medicine, medicine.medical_specialty, Biology
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