publication . Article . 2007

Disappearance of interfering alkali-metal adducted peaks from matrix-assisted laser desorption/ionization mass spectra of peptides with serine addition toα-cyano-4-hydroxycinnamic acid matrix

Mitsuo Takayama; Takashi Nishikaze;
Restricted
  • Published: 20 Sep 2007 Journal: Rapid Communications in Mass Spectrometry, volume 21, pages 3,345-3,351 (issn: 0951-4198, eissn: 1097-0231, Copyright policy)
  • Publisher: Wiley
Abstract
It has been described that ion yield in both positive- and negative-ion matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) of peptides is often inhibited by trace amounts of alkali metals and that the MALDI mass spectra are contaminated by the interfering peaks originating from traces of alkali metals, even when sample preparation is carefully performed. Addition of serine to the commonly used MALDI matrix alpha-cyano-4-hydroxycinnamic acid (CHCA) significantly improved and enhanced the signals of both protonated and deprotonated peptides, [M+H](+) and [M-H](-). The addition of serine to CHCA matrix eliminated the alkali-metal ion adducts, ...
Subjects
free text keywords: Matrix-assisted laser desorption/ionization, alpha-Cyano-4-hydroxycinnamic acid, chemistry.chemical_compound, chemistry, Adduct, Tandem mass spectrometry, Desorption, Ion, Protonation, Nuclear chemistry, Mass spectrum, Analytical chemistry
Related Organizations
Any information missing or wrong?Report an Issue