publication . Article . 2000

Construction of a binary bacterial artificial chromosome library of Petunia inflata and the isolation of large genomic fragments linked to the self-incompatibility (S-) locus

Andrew G McCubbin; Carmen Zuniga; Teh-hui Kao;
  • Published: 01 Oct 2000 Journal: Genome, volume 43, pages 820-826 (issn: 0831-2796, eissn: 1480-3321, Copyright policy)
  • Publisher: Canadian Science Publishing
<jats:p> The Solanaceae family of flowering plants possesses a type of self-incompatibility mechanism that enables the pistil to reject self pollen but accept non-self pollen for fertilization. The pistil function in this system has been shown to be controlled by a polymorphic gene at the S-locus, termed the S-RNase gene. The pollen function is believed to be controlled by another as yet unidentified polymorphic gene at the S-locus, termed the pollen S-gene. As a first step in using a functional genomic approach to identify the pollen S-gene, a genomic BAC (bacterial artificial chromosome) library of the S<jats:sub>2</jats:sub>S<jats:sub>2</jats:sub> genotype of...
Medical Subject Headings: food and beverages
free text keywords: Biotechnology, Genetics, Molecular Biology, General Medicine
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