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Pathogens
Other literature type . Article . 2022 . Peer-reviewed
License: CC BY
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Pathogens
Article . 2022
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Detection and Multigene Typing of ‘Candidatus Phytoplasma solani’-Related Strains Infecting Tomato and Potato Plants in Different Regions of Turkey

Authors: Behçet Kemal Çağlar; Eray Şimşek;

Detection and Multigene Typing of ‘Candidatus Phytoplasma solani’-Related Strains Infecting Tomato and Potato Plants in Different Regions of Turkey

Abstract

‘Candidatus Phytoplasma solani’ (‘Ca. P. solani’) is a crop pathogen that is a member of the 16SrXII-A ribosomal subgroup. It is also known as stolbur phytoplasma and causes yield losses in several important crops, especially in Solanaceous crops. Different strains of the pathogen are regularly reported all over the world, particularly in the Mediterranean region. In this study, the determination of genetic diversity for the pathogen infecting tomatoes and potatoes was carried out by using multilocus sequence typing analysis for the Tuf, SecY, and Vmp1 genes to gain insight into the epidemiology of ‘Ca. P. solani’ in Turkey. Genetic diversity of the phytoplasmas was investigated by sequence-based phylogenetic analyses and in silico RFLP analysis of related genes. It was determined that all ‘Ca. P. solani’-related strains infecting tomatoes and potatoes were tuf-b, which is linked to field bindweed (Convolvulus arvensis L.). Tomato or potato-infecting ‘Ca. P. solani’-related strains showed similarities with each other; however, the isolates collected from different plants showed genetic differences in terms of the SecY gene. This study indicates that the highest genetic variability of collected samples was found in the Vmp1 gene. RsaI-RFLP analysis of TYPH10F/R amplicons showed that potato-infecting ‘Ca. P. solani’-related strains were found to be similar to some existing V types. However, the V-type of tomato-infecting isolates is not similar to any previously reported V-type. The results indicate that there could be an important genetic diversity of ‘Ca. P. solani’-related phytoplasmas in Turkey. This could indicate various ways in which the pathogen has adapted to the two host plants as a consequence of the various Vmp1 gene rearrangements seen in these two plant hosts. Obtained results also indicate that the epidemiology of ‘Ca. P. solani’-related phytoplasmas in the tomato and potato agroecosystem may be better understood with the use of molecular data on the complex of vmp-types.

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Keywords

Microbiology (medical), ‘<i>Candidatus</i> Phytoplasma solani’; potato; tomato; <i>Sec</i>Y; <i>Vmp</i>1; <i>Tuf</i>; <i>16SrDNA</i>, General Immunology and Microbiology, <i>Sec</i>Y, R, <i>Vmp</i>1, tomato, Infectious Diseases, potato, Medicine, Immunology and Allergy, ‘<i>Candidatus</i> Phytoplasma solani’, <i>Tuf</i>, Molecular Biology

57 references, page 1 of 6

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6. S¸ims¸ek, E.; Güldür, M. Detection and molecular characterization of phytoplasmas based on 16s rdna gene region by phylogenetic and in silico rflp analysis of local grapevine cultivars in S¸anlıurfa and Adıyaman. Harran Tarım Ve Gıda Bilim. Derg. 2021, 25, 204-213. [CrossRef]

7. Çag˘ lar, B.K.; S¸ims¸ek, E.; Dikilitas, M.; Bertaccini, A. Characterization of 'Candidatus Phytoplasma Solani' associated with a maize leaf reddening disease in Turkey. J. Phytopathol. 2021, 169, 658-666. [CrossRef]

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    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Top 10%
Average
Average
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