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Publication . Article . 2005

Shared reactivity of Vδ2neg γδ T cells against cytomegalovirus-infected cells and tumor intestinal epithelial cells

Halary, Franck; Pitard, Vincent; Dlubek, Dorota; Krzysiek, Roman; de la Salle, Henri; Merville, Pierre; Dromer, Claire; +3 Authors
Open Access   English  
Published: 16 May 2005 Journal: The Journal of Experimental Medicine, volume 201, issue 10, pages 1,567-1,578 (issn: 0022-1007, eissn: 1540-9538, Copyright policy )
Publisher: The Rockefeller University Press
Abstract
Long-lasting expansion of Vδ2neg γδ T cells is a hallmark of cytomegalovirus (CMV) infection in kidney transplant recipients. The ligands of these cells and their role remain elusive. To better understand their immune function, we generated γδ T cell clones from several transplanted patients. Numerous patient Vδ1+, Vδ3+, and Vδ5+ γδ T cell clones expressing diverse Vγ chains, but not control Vγ9Vδ2+ T clones, displayed strong reactivity against CMV-infected cells, as shown by their production of tumor necrosis factor-α. Vδ2neg γδ T lymphocytes could also kill CMV-infected targets and limit CMV propagation in vitro. Their anti-CMV reactivity was specific for this virus among herpesviridae and required T cell receptor engagement, but did not involve major histocompatibility complex class I molecules or NKG2D. Vδ2neg γδ T lymphocytes expressed receptors essential for intestinal homing and were strongly activated by intestinal tumor, but not normal, epithelial cell lines. High frequencies of CMV- and tumor-specific Vδ2neg γδ T lymphocytes were found among patients' γδ T cells. In conclusion, Vδ2neg γδ T cells may play a role in protecting against CMV and tumors, probably through mucosal surveillance of cellular stress, and represent a population that is largely functionally distinct from Vγ9Vδ2+ T cells.
Subjects by Vocabulary

Microsoft Academic Graph classification: IL-2 receptor Interleukin 21 NKG2D Biology Immune system Major histocompatibility complex biology.protein T cell medicine.anatomical_structure medicine Cytotoxic T cell Streptamer Immunology

Subjects

Article, Immunology, Immunology and Allergy

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