publication . Article . 2014

Subcellular metal imaging identifies dynamic sites of Cu accumulation in Chlamydomonas.

Anne Hong-Hermesdorf; Marcus Miethke; Sean D. Gallaher; Janette Kropat; Sheel C. Dodani; Jefferson Y. Chan; Dulmini Barupala; Dylan W. Domaille; Dyna I. Shirasaki; Joseph A. Loo; ...
Open Access
  • Published: 26 Oct 2014
  • Publisher: eScholarship, University of California
  • Country: United States
Abstract
We identified a Cu-accumulating structure with a dynamic role in intracellular Cu homeostasis. During Zn limitation, Chlamydomonas reinhardtii hyperaccumulates Cu, a process dependent on the nutritional Cu sensor CRR1, but it is functionally Cu deficient. Visualization of intracellular Cu revealed major Cu accumulation sites coincident with electron-dense structures that stained positive for low pH and polyphosphate, suggesting that they are lysosome-related organelles. Nano-secondary ion MS showed colocalization of Ca and Cu, and X-ray absorption spectroscopy was consistent with Cu(+) accumulation in an ordered structure. Zn resupply restored Cu homeostasis con...
Persistent Identifiers
Subjects
free text keywords: Lysosomes, Chlamydomonas reinhardtii, Polyphosphates, Cations, Divalent, Copper, Zinc, Isotopes, Plastocyanin, Transcription Factors, Gene Expression Profiling, Isotope Labeling, Homeostasis, Hydrogen-Ion Concentration, Molecular Imaging, Transcriptome, Biochemistry & Molecular Biology, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology, Cell Biology, Molecular Biology, Article, Bioinorganic chemistry, Chemistry, Plastocyanin, Intracellular, Divalent, chemistry.chemical_classification, Zinc, chemistry.chemical_element, Chlamydomonas, biology.organism_classification, biology, Polyphosphate, chemistry.chemical_compound, Biophysics, Inorganic chemistry, Chlamydomonas reinhardtii
Funded by
NIH| Detroit Cardiovascular Training Program
Project
  • Funder: National Institutes of Health (NIH)
  • Project Code: 5T32HL120822-02
  • Funding stream: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
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