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Antibody Characterization Report for Transmembrane protein 106B

Authors: Ayoubi, Riham; Fotouhi, Maryam; Ryan, Joel; Reintsch, Wolfgang; Durcan, Thomas M.; Brown, Claire M.; McPherson, Peter S.; +1 Authors

Antibody Characterization Report for Transmembrane protein 106B

Abstract

Head-to-head comparison of available commercial antibodies against Transmembrane protein 106B by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The workflow and data collected in this study were used to create an article that was published on F1000, DOI: 10.12688/f1000research.131333.1. The following study was funded in part by Genome Québec's Genomics Integration Program, awarded to the research laboratory of Peter S. McPherson. This work was supported in part by the Michael J. Fox Foundation for Parkinson's research.

Keywords

amyotrophic lateral sclerosis, antibody characterization, TMEM106B, Parkinson's disease, Transmembrane protein 106B, Q9NUM4, antibody validation, ALS-RAP

Laflamme, C., et al., Opinion: Independent third-party entities as a model for validation of commercial antibodies. N Biotechnol, 2021. 65: p. 1-8 DOI: 10.1016/j.nbt.2021.07.001.

Laflamme, C., et al., Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Elife, 2019. 8 DOI: 10.7554/eLife.48363.

Ghandi, M., et al., Next-generation characterization of the Cancer Cell Line Encyclopedia. Nature, 2019. 569(7757): p. 503-508 DOI: 10.1038/s41586-019-1186-3.

2021 DOI: https://doi.org/10.5281/zenodo.5717510.

Ayoubi, R., et al., Antibody screening by Immunoprecitation. 2021 DOI: https://doi.org/10.5281/zenodo.5717516.

Alshafie, W., P. McPherson, and C. Laflamme, Antibody screening by Immunofluorescence. 2021 DOI: https://doi.org/10.5281/zenodo.5717498.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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