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Publication . Article . 2015

Antibiogram Typing of Biofield Treated Multidrug Resistant Strains of Staphylococcus Species

Trivedi, Mahendra Kumar; Branton, Alice; Trivedi, Dahryn; Nayak, Gopal; Gangwar, Mayank; Jana, Snehasis;
Open Access

Antimicrobial resistance is a global health issue in the developing countries. This study was carried out to evaluate the impact of Mr. Trivedi’s biofield energy treatment on multidrug resistant (MDR) clinical lab isolates (LSs) of Staphylococcus species viz. Staphylococcus haemolyticus (LS 18), Staphylococcus epidermidis (LS 21), and Staphylococcus aureus (LS 30). Each strain was divided into the two groups i.e. control and treated. The control and treated groups were analyzed for the antimicrobial susceptibility pattern, minimum inhibitory concentration (MIC), biochemical analysis and biotype number using MicroScan Walk-Away® system. The analysis was done on day 10 after biofield treatment and compared with the control group. The sensitivity of erythromycin was improved from resistant to susceptible, while levofloxacin sensitivity was also improved from intermediate to susceptible in LS 21 isolate. The MIC results showed a decrease in the concentrations of ceftriaxone, erythromycin, imipenem, and levofloxacin antimicrobials in LS 21 as compared to the control. Linezolid and vancomycin also showed decrease in MIC as compared to the control in LS 30. Overall, 20.69% antimicrobials showed decrease in MIC value out of the tested twenty-nine after biofield treatment in Staphylococcus species. The biochemical study showed a 25% alteration in biochemical reactions as compared to the control. A significant change was reported in biotype numbers for all the three strains of MDR Staphylococcus species after biofield treatment as compared to the respective control group. On the basis of changed biotype number (306366) after biofield treatment in LS 18, the new organism was identified as Staphylococcus simulans with respect to the control species i.e. Staphylococcus haemolyticus (302302). The control group of S. epidermidis and S. aureus showed biotype number as 303064 and 757153 respectively. After biofield treatment, LS 21 and LS 30 isolates showed altered biotype number as 307064 and 317153 respectively. Overall, results conclude that biofield treatment could be used as complementary and alternative treatment strategy against multidrug resistant strains of Staphylococcus species with improved sensitivity and reduced MIC values of antimicrobial.

Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana. Antibiogram Typing of Biofield Treated Multidrug Resistant Strains of Staphylococcus Species. American Journal of Life Sciences. Vol. 3, No. 5, 2015, pp. 369-374. doi: 10.11648/j.ajls.20150305.16


Staphylococcus haemolyticus, Staphylococcus epidermidis, Staphylococcus aureus, Biofield Energy Treatment, Multidrug-Resistant, Antibiogram, Biotyping, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology

31 references, page 1 of 4

Heilmann C, Peters G (2000) Biology and pathogenicity of Staphylococcus epidermidis. Gram-positive pathogens ASM Press, Washington, D. C.

Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn Jr (1997) Color atlas and textbook of diagnostic microbiology. (5thedn), Lippincott, Philadelphia.

[3] Falcone M, Campanile F, Giannella M, Borbone S, Stefani S, et al. (2007) Staphylococcus haemolyticus endocarditis: Clinical and microbiologic analysis of 4 cases. Diagn Microbiol Infect Dis 57: 325-331.

John JF, Harvin A (2007) History and evolution of antibiotic resistance in coagulase negative staphylococci: Susceptibility profiles of new anti-staphylococcal agents. Ther Clin Risk Manag 3: 1143-1152. [OpenAIRE]

Favre B, Hugonnet S, Correa S, Sax H, Rohner P, et al. (2005) Nosocomial bacteremia: clinical significance of a single blood culture positive for coagulase-negative staphylococci. Infect Control Hosp Epidemiol 26: 697-702. [OpenAIRE]

Vuong C, Otto M (2002) Staphylococcus epidermidis infections. Microbes Infect 4: 481-489.

Rupp ME, Crossley KB, Archer GL (1997) The Staphylococci in human disease. Infections of intravascular catheters and vascular devices. New York, Churchill Livingstone.

Agvald-Ohman C, Lund B, Edlund C (2004) Multiresistant coagulase-negative staphylococci disseminate frequently between intubated patients in a multidisciplinary intensive care unit. Critic Care 8: 42-47.

Dinges MM, Orwin PM, Schlievert PM (2000) Exotoxins of Staphylococcus aureus. Clin Microbiol Rev 13: 16-33. [OpenAIRE]

[10] Trivedi MK, Patil S, Shettigar H, Gangwar M, Jana S (2015) An effect of biofield treatment on multidrug-resistant Burkholderia cepacia: A multihost pathogen. J Trop Dis 3: 167.