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Antibody Characterization Report for Serine/threonine-protein kinase TBK1
Alshafie, Walaa; Fotouhi, Maryam; Shlaifer, Irina; Durcan, Thomas M.; McPherson, Peter S.; Laflamme, Carl;
Alshafie, Walaa; Fotouhi, Maryam; Shlaifer, Irina; Durcan, Thomas M.; McPherson, Peter S.; Laflamme, Carl;
Antibody Characterization Report for Serine/threonine-protein kinase TBK1
Head to head comparison of available commercial antibodies against Serine/threonine-protein kinase TBK1 by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The workflow and data collected in this study was used to create an article published on F1000, DOI: 10.12688/f1000research.124632.2. This work is part of the ALS-Reproducible Antibody Platform (ALS-RAP). ALS-RAP was created as a public-private partnership by three leading ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.
Serine/threonine-protein kinase TBK1, T2K, NF-kappa-B-activating kinase, TANK-binding kinase 1, antibody characterization, TBK1, Q9UHD2, NAK, antibody validation, Amyotrophic lateral sclerosis, ALS-RAP
Serine/threonine-protein kinase TBK1, T2K, NF-kappa-B-activating kinase, TANK-binding kinase 1, antibody characterization, TBK1, Q9UHD2, NAK, antibody validation, Amyotrophic lateral sclerosis, ALS-RAP
Laflamme, C., et al., Opinion: Independent third-party entities as a model for validation of commercial antibodies. N Biotechnol, 2021. 65: p. 1-8 DOI: 10.1016/j.nbt.2021.07.001.
Laflamme, C., et al., Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Elife, 2019. 8 DOI: 10.7554/eLife.48363.
Wang, M., et al., Version 4.0 of PaxDb: Protein abundance data, integrated across model organisms, tissues, and cell-lines. Proteomics, 2015. 15(18): p. 3163-8 DOI: 10.1002/pmic.201400441.
Cell, 2020. 180(2): p. 387-402 e16 DOI: 10.1016/j.cell.2019.12.023.
Shlaifer, I., et al. Generation of Knockout Cell Lines Using CRISPR-Cas9 Technology.
February 24, 2020; Available from: https://zenodo.org/record/3738361#.YIyeDu2SlaR.
2021 DOI: https://doi.org/10.5281/zenodo.5717510.
Ayoubi, R., et al., Antibody screening by Immunoprecitation. 2021 DOI: https://doi.org/10.5281/zenodo.5717516.
Alshafie, W., P. McPherson, and C. Laflamme, Antibody screening by Immunofluorescence. 2021 DOI: https://doi.org/10.5281/zenodo.5717498.
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).1 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average views 343 downloads 222 citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).1 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average - 343views222downloads
citations
Citations provided by BIP!
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
Citations provided by BIP!popularityPopularity provided by BIP!
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
Popularity provided by BIP!influenceInfluence provided by BIP!
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
Influence provided by BIP!impulseImpulse provided by BIP!
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
Impulse provided by BIP!viewsViews provided by UsageCounts
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Average
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343
222
Head to head comparison of available commercial antibodies against Serine/threonine-protein kinase TBK1 by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The workflow and data collected in this study was used to create an article published on F1000, DOI: 10.12688/f1000research.124632.2. This work is part of the ALS-Reproducible Antibody Platform (ALS-RAP). ALS-RAP was created as a public-private partnership by three leading ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.