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Antibody Characterization Report for Vesicle-associated membrane protein-associated protein B/C (VAPB)
Fotouhi, Maryam; Alshafie, Walaa; Shlaifer, Irina; Ayoubi, Riham; Durcan, Thomas M.; McPherson, Peter S.; Laflamme, Carl;
Fotouhi, Maryam; Alshafie, Walaa; Shlaifer, Irina; Ayoubi, Riham; Durcan, Thomas M.; McPherson, Peter S.; Laflamme, Carl;
Antibody Characterization Report for Vesicle-associated membrane protein-associated protein B/C (VAPB)
Head-to-head comparison of available commercial antibodies against Vesicle-associated membrane protein-associated protein B/C (VAPB) by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The following study was funded in part by Genome Québec's Genomics Integration Program, awarded to the research laboratory of Peter S. McPherson. This work was supported by the ALS-Reproducible Antibody Platform (ALS-RAP), a public-private partnership established by three prominent ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.
amyotrophic lateral sclerosis, antibody characterization, YCharOS, Vesicle-associated membrane protein-associated protein B/C, VAMP-associated protein B/C, O95292, antibody validation, VAPB, ALS-RAP
amyotrophic lateral sclerosis, antibody characterization, YCharOS, Vesicle-associated membrane protein-associated protein B/C, VAMP-associated protein B/C, O95292, antibody validation, VAPB, ALS-RAP
Laflamme, C., et al., Opinion: Independent third-party entities as a model for validation of commercial antibodies. N Biotechnol, 2021. 65: p. 1-8 DOI: 10.1016/j.nbt.2021.07.001.
Laflamme, C., et al., Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Elife, 2019. 8 DOI: 10.7554/eLife.48363.
Wang, M., et al., Version 4.0 of PaxDb: Protein abundance data, integrated across model organisms, tissues, and cell-lines. Proteomics, 2015. 15(18): p. 3163-8 DOI: 10.1002/pmic.201400441.
Cell, 2020. 180(2): p. 387-402 e16 DOI: 10.1016/j.cell.2019.12.023.
DepMap, Broad. 2019.
Nicouleau, M., et al., Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology. 2020 DOI: 10.5281/zenodo.3875777.
2021 DOI: https://doi.org/10.5281/zenodo.5717510.
Ayoubi, R., et al., Antibody screening by Immunoprecitation. 2021 DOI: https://doi.org/10.5281/zenodo.5717516.
Alshafie, W., P. McPherson, and C. Laflamme, Antibody screening by Immunofluorescence. 2021 DOI: https://doi.org/10.5281/zenodo.5717498.
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).0 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average views 126 downloads 101 citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).0 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average - 126views101downloads
citations
Citations provided by BIP!
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
Citations provided by BIP!popularityPopularity provided by BIP!
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
Popularity provided by BIP!influenceInfluence provided by BIP!
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
Influence provided by BIP!impulseImpulse provided by BIP!
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
Impulse provided by BIP!viewsViews provided by UsageCounts
Views provided by UsageCountsdownloadsDownloads provided by UsageCounts
Downloads provided by UsageCounts 0
Average
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Average
126
101
Head-to-head comparison of available commercial antibodies against Vesicle-associated membrane protein-associated protein B/C (VAPB) by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The following study was funded in part by Genome Québec's Genomics Integration Program, awarded to the research laboratory of Peter S. McPherson. This work was supported by the ALS-Reproducible Antibody Platform (ALS-RAP), a public-private partnership established by three prominent ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.