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antibody characterization report for sequestosome 1

Authors: Ayoubi, Riham; Alshafie, Walaa; Shlaifer, Irina; Durcan, Thomas M.; McPherson, Peter S.; Laflamme, Carl;

antibody characterization report for sequestosome 1

Abstract

Head to head comparison of available commercial antibodies against Sequestosome-1 by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The workflow and data collected in this study was used to create an article published on F1000, DOI: 10.12688/f1000research.132628.1. This work is part of the ALS-Reproducible Antibody Platform (ALS-RAP). ALS-RAP was created as a public-private partnership by three leading ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.

Keywords

antibody characterization, p60, p62, Ubiquitin-binding protein p62, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, EBIAP, Amyotrophic lateral sclerosis, ALS-RAP, sqstm1, EBI3-associated protein of 60 kDa, Q13501, SQSTM1, Sequestosome-1, antibody validation

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