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Antibody Characterization Report for Charged multivesicular body protein 2b

Authors: Fotouhi, Maryam; Alshafie, Walaa; shlaifer, Irina; Ayoubi, Riham; Durcan, Thomas M.; McPherson, Peter S.; Laflamme, Carl;

Antibody Characterization Report for Charged multivesicular body protein 2b

Abstract

Head-to-head comparison of available commercial antibodies against Charged multivesicular body protein 2b by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The workflow and data collected in this study was used to create an article published on F1000, DOI: 10.12688/f1000research.139755.1. Work reported in this report was supported in part by the ALS-Reproducible Antibody Platform (ALS-RAP). ALS-RAP was created as a public-private partnership by three leading ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.

Keywords

Vps2-2, amyotrophic lateral sclerosis, antibody characterization, YCharOS, Chromatin-modifying protein 2b, Vacuolar protein sorting-associated protein 2-2, CHMP2B, Q9UQN3, antibody validation, ALS-RAP, Charged multivesicular body protein 2b

Laflamme, C., et al., Opinion: Independent third-party entities as a model for validation of commercial antibodies. N Biotechnol, 2021. 65: p. 1-8 DOI: 10.1016/j.nbt.2021.07.001.

Laflamme, C., et al., Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Elife, 2019. 8 DOI: 10.7554/eLife.48363.

Wang, M., et al., Version 4.0 of PaxDb: Protein abundance data, integrated across model organisms, tissues, and cell-lines. Proteomics, 2015. 15(18): p. 3163-8 DOI: 10.1002/pmic.201400441.

Cell, 2020. 180(2): p. 387-402 e16 DOI: 10.1016/j.cell.2019.12.023.

DepMap, Broad. 2019.

Nicouleau, M., et al., Generation of Knockout Cell Lines Using CRISPR-Cas9 and ddPCR Technology. 2020 DOI: 10.5281/zenodo.3875777.

2021 DOI: https://doi.org/10.5281/zenodo.5717510.

Ayoubi, R., et al., Antibody screening by Immunoprecitation. 2021 DOI: https://doi.org/10.5281/zenodo.5717516.

Alshafie, W., P. McPherson, and C. Laflamme, Antibody screening by Immunofluorescence. 2021 DOI: https://doi.org/10.5281/zenodo.5717498.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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