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Antibody Characterization Report for Spatacsin

Authors: Ayoubi, Riham; Fotouhi, Maryam; Ryan, Joel; Reintsch, Wolfgang; Durcan, Thomas M.; Brown, Claire M.; McPherson, Peter S.; +1 Authors

Antibody Characterization Report for Spatacsin

Abstract

Head-to-head comparison of available commercial antibodies against Spatacsin by immunoblot (Western blot), immunoprecipitation and immunofluorescence. The following study was funded in part by Genome Québec's Genomics Integration Program, awarded to the research laboratory of Peter S. McPherson. This work was supported by the ALS-Reproducible Antibody Platform (ALS-RAP), a public-private partnership established by three prominent ALS charities - the ALS Association (USA), the Motor Neurone Disease Association (UK), and the ALS Society of Canada.

Keywords

Spastic paraplegia 11 protein, amyotrophic lateral sclerosis, antibody characterization, SPG11, Spatacsin, Colorectal carcinoma-associated protein, antibody validation, ALS-RAP, Q96JI7

Laflamme, C., et al., Opinion: Independent third-party entities as a model for validation of commercial antibodies. N Biotechnol, 2021. 65: p. 1-8 DOI: 10.1016/j.nbt.2021.07.001.

Laflamme, C., et al., Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. Elife, 2019. 8 DOI: 10.7554/eLife.48363.

Ghandi, M., et al., Next-generation characterization of the Cancer Cell Line Encyclopedia. Nature, 2019. 569(7757): p. 503-508 DOI: 10.1038/s41586-019-1186-3.

2021 DOI: https://doi.org/10.5281/zenodo.5717510.

Ayoubi, R., et al., Antibody screening by Immunoprecitation. 2021 DOI: https://doi.org/10.5281/zenodo.5717516.

Alshafie, W., P. McPherson, and C. Laflamme, Antibody screening by Immunofluorescence. 2021 DOI: https://doi.org/10.5281/zenodo.5717498.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
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