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Publication . Article . 2018

Detection of Escherichia Coli hlyA gene and Staphylococcus aureus Sea gene in raw milk of buffaloes using RT-PCR technique in AL- Qadisiyah province

Jenan Nadhim Sadeq; Kh.H. Fahed; H.J. Hassan;
Open Access
Published: 01 Jun 2018 Journal: Iraqi Journal of Veterinary Sciences, volume 32, pages 87-91 (eissn: 2071-1255, Copyright policy )
Publisher: University of Mosul

The aim of this study was to determines the prevalence of virulence gene hemolysin A (hly A) Escherichia coli and Staphylococcal enterotoxins (sea) in Staphylococcus aureus in raw milk buffaloes. In molecular laboratory, real-time polymerase chain reaction (RT-PCR) technique has been performed for 24 samples which have been taken randomly from Buffaloes milk, using primers of high specificity for Escherichia coli hlyA gene and Staphylococcus aureus Sea genes. The results showed different degrees of the studied genes activities. Four out of 24 samples represented S. aureus Sea gene (16.6%) whereas 16 out of 24 samples represented E. coli hlyA gene (66.6%). this study concluded that buffaloes milk might be a source of contamination with pathogenic bacteria of virulent genes which may have different levels of activities.

Subjects by Vocabulary

Microsoft Academic Graph classification: Real-time polymerase chain reaction Staphylococcus aureus medicine.disease_cause medicine Escherichia coli Microbiology Raw milk Gene Biology

Library of Congress Subject Headings: lcsh:Veterinary medicine lcsh:SF600-1100

Medical Subject Headings: food and beverages


real, time polymerase chain reaction, escherichia coli, hlya gene, staphylococcus aureus, sea gene

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Iraqi Journal of Veterinary Sciences
License: cc-by
Providers: UnpayWall