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Publication . Article . Other literature type . 2020

Vector competence of anthropophilic mosquitoes for a new mesonivirus in Senegal

Alioune Gaye; Moussa Moïse Diagne; El Hadji Ndiaye; Marie Henriette Dior Ndione; Martin Faye; Cheikh Talla; Gamou Fall; +7 Authors
Open Access   English  
Published: 28 Feb 2020
Country: France
Abstract
International audience; The mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named Dianke virus (DKV) from common mosquitoes from eastern Senegal. Actually, their vector competence for this newly described DKV is unknown. We, therefore, estimated the vector competence of Culex tritaeniorhynchus, Culex quinquefasciatus, Aedes aegypti, and Anopheles gambiae mosquitoes collected in Senegal for DKV using oral infection. Whole bodies, legs/wings, and saliva samples were tested for DKV by RT-PCR to estimate infection, dissemination, and transmission rates. The infectivity of virus particles in the saliva was confirmed by infecting C6/36 cells. Virus transmission rates were up to 95.45% in Culex tritaeniorhynchus, 28% in Cx. quinquefasciatus and 9.09% in Aedes aegypti. Viral particles in the saliva were confirmed infectious by C6/36 cell culture. An. gambiae was able to disseminate DKV only at 20 days post-infection. This study shows that Culex mosquitoes are more competent than Ae. aegypti for DKV, while Anopheles gambiae is not likely a competent vector.
Subjects by Vocabulary

Medical Subject Headings: parasitic diseases fungi viruses virus diseases

Microsoft Academic Graph classification: Arbovirus medicine.disease medicine Virology Culex quinquefasciatus biology.organism_classification biology Aedes aegypti Virus Culex tritaeniorhynchus Nidovirales Infectivity Anopheles gambiae

Subjects

Vector competence, Dianke virus, Culex, Aedes, Anopheles, Aedes, Anopheles, Culex, Dianke virus, Vector competence., [SDV]Life Sciences [q-bio], Virology, Infectious Diseases, Drug Discovery, General Medicine, Immunology, Microbiology, Parasitology, Epidemiology, Article, Vector competence

Funded by
ANR| CHANCIRA
Project
CHANCIRA
Environmental changes, goods and people movements: from invasion of reservoirs to emergence of human diseases. Case study of the black rat in the Senegal-Mali boundaries._x000D_
  • Funder: French National Research Agency (ANR) (ANR)
  • Project Code: ANR-11-CEPL-0010
,
ANR| ENEMI
Project
ENEMI
Evolutionary consequences of Natural Enemies in Major biological Invasions : the role of parasites in the invasion success of two commensal rodents
  • Funder: French National Research Agency (ANR) (ANR)
  • Project Code: ANR-11-JSV7-0006