publication . Preprint . Article . 2018

Presence of causative mutations affecting prolificacy in the Noire du Velay and Mouton Vendéen sheep breeds

Chantepie, L.; Bodin, L.; Sarry, J.; Woloszyn, F.; Ruesche, J.; Drouilhet, L.; Fabre, S.;
Open Access
  • Published: 11 Jul 2018
  • Publisher: bioRxiv
  • Country: France
Abstract
AbstractFor many decades, prolificacy has been selected in meat sheep breeds as a polygenic trait but with limited genetic gain. However, the discovery of major genes affecting prolificacy has changed the way of selection for some ovine breeds implementing gene-assisted selection as in the French Lacaune and Grivette meat breeds, or in the Spanish Rasa Aragonesa breed. Based on statistical analysis of litter size parameters from 34 French meat sheep populations, we suspected the segregation of a mutation in a major gene affecting prolificacy in the Noire du Velay and in the Mouton Vendéen breeds exhibiting a very high variability of the litter size. After the genotyping of mutations known to be present in French sheep breeds, we discovered the segregation of the FecLL mutation at the B4GALNT2 locus and the FecXGr mutation at the BMP15 locus in Noire du Velay and Mouton Vendéen, respectively. The frequency of ewes carrying FecLL in the Noire du Velay population was estimated at 21.2% and the Mouton Vendéen ewes carrying FecXGr at 10.3%. The estimated mutated allele effect of FecLL and FecXGron litter size at +0.4 and +0.3 lamb per lambing in Noire du Velay and Mouton Vendéen, respectively. Due to the fairly high frequency and the rather strong effect of the FecLL and FecXGr prolific alleles, specific management programmes including genotyping should be implemented for a breeding objective of prolificacy adapted to each of these breeds.
Subjects
free text keywords: General Veterinary, Animal Science and Zoology, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, ovine, major gene, prolificacy, BMP15, B4GALNT2, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Autre (Sciences du Vivant), ovine;major gene;prolificacy;BMP15;B4GALNT2, Locus (genetics), Genetic gain, Allele, Genotyping, Breed, Biology, Domestic sheep reproduction, Veterinary medicine, Major gene, Population, education.field_of_study, education
32 references, page 1 of 3

Amer, P.R., Bodin, L., 2006. Quantitative genetic selection for twinning rate in ewes. N.Z. Soc. Anim. Product.

Bodin, L., Di Pasquale, E., Fabre, S., Bontoux, M., Monget, P., Persani, L., Mulsant, P., 2007. A novel mutation in the bone morphogenetic protein 15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep. Endocrinology 148, 393-400.

Bodin, L., Elsen, J.M., Station d'amelioration genetique des animaux, 1989. Variability of litter size of french sheep breeds following natural or induced ovulation. Anim. Product. 535-541.

Bodin, L., Raoul, J., Demars, J., Drouilhet, L., Mulsant, P., Sarry, J., Tabet, C., TosserKlopp, G., Fabre, S., Boscher, M.Y., 2011. Etat des lieux et gestion pratique des gènes d'ovulation détectés dans les races ovines françaises. 18èmes Rencontres Recherches Ruminants. Institut de l'Elevage, Paris, France, pp. 393-400.

Bradford, G.E., 1985. Selection for litter size. In: Land, R.B., Robinson, D.W. (Eds.), Genetics of Reproduction in Sheep. Butterworths, London, pp. 3-18.

Cottle, D.J., Gilmour, A.R., Pabiou, T., Amer, P.R., Fahey, A.G., 2016. Genetic selection for increased mean and reduced variance of twinning rate in Belclare ewes. J. Anim. Breed. Genet. 133, 126-137.

Davis, G.H., Montgomery, G.W., Allison, A.J., Kelly, R.W., Bray, A.R., 1982. Segregation of a major gene influencing fecundity in progeny of Booroola sheep. N.Z. J. Agric. Res. 25, 525-529.

Demars, J., Fabre, S., Sarry, J., Rossetti, R., Gilbert, H., Persani, L., Tosser-Klopp, G., Mulsant, P., Nowak, Z., Drobik, W., Martyniuk, E., Bodin, L., 2013. Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep. PLos Genet. 9, e1003482. [OpenAIRE]

Drouilhet, L., Lecerf, F., Bodin, L., Fabre, S., Mulsant, P., 2009. Fine mapping of the FecL locus influencing prolificacy in Lacaune sheep. Anim. Genet. 40, 804-812. [OpenAIRE]

Drouilhet, L., Mansanet, C., Sarry, J., Tabet, K., Bardou, P., Woloszyn, F., Lluch, J., Harichaux, G., Viguié, C., Monniaux, D., Bodin, L., Mulsant, P., Fabre, S., 2013. The highly prolific phenotype of Lacaune sheep is associated with an ectopic expression of the B4GALNT2 gene within the ovary. PLos Genet. 9, e1003809.

Fabre, S., Pierre, A., Mulsant, P., Bodin, L., Di Pasquale, E., Persani, L., Monget, P., Monniaux, D., 2006. Regulation of ovulation rate in mammals: contribution of sheep genetic models. Reproduct. Biol. Endocrinol. 4, 20. [OpenAIRE]

Fathallah, S., Alabart, J.L., Bodin, L., Jimenez-Hernando, M.A., Lahoz, B., Fantova, E., David, I., Jurado, J.J., 2016. Relaciones entre los efectos del gen BMP15 y los efectos poligénicos sobre la prolificidad en la raza ovina Rasa Aragonesa. Informacion Tecnica Economica Agraria 112, 45-56.

Galloway, S.M., McNatty, K.P., Cambridge, L.M., Laitinen, M.P.E., Juengel, J.L., Jokiranta, T.S., McLaren, R.J., Luiro, K., Dodds, K.G., Montgomery, G.W., Beattie, A.E., Davis, G.H., Ritvos, O., 2000. Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nat. Genet. 25, 279-283. [OpenAIRE]

Gianola, D., 1982. Assortative mating and the genetic correlation. Theor. Appl. Genet. 62, 225-231.

Gilmour, A.R., Gogel, B.J., Cullis, B.R., Welham, S.J., Thompson, R., 2014. ASReml User Guide Release 4.1 Functional Specification. VSN International Ltd, Hemel Hempstead, United Kingdom. http://www.vsni.co.uk.

32 references, page 1 of 3
1 research outcomes, page 1 of 1
Abstract
AbstractFor many decades, prolificacy has been selected in meat sheep breeds as a polygenic trait but with limited genetic gain. However, the discovery of major genes affecting prolificacy has changed the way of selection for some ovine breeds implementing gene-assisted selection as in the French Lacaune and Grivette meat breeds, or in the Spanish Rasa Aragonesa breed. Based on statistical analysis of litter size parameters from 34 French meat sheep populations, we suspected the segregation of a mutation in a major gene affecting prolificacy in the Noire du Velay and in the Mouton Vendéen breeds exhibiting a very high variability of the litter size. After the genotyping of mutations known to be present in French sheep breeds, we discovered the segregation of the FecLL mutation at the B4GALNT2 locus and the FecXGr mutation at the BMP15 locus in Noire du Velay and Mouton Vendéen, respectively. The frequency of ewes carrying FecLL in the Noire du Velay population was estimated at 21.2% and the Mouton Vendéen ewes carrying FecXGr at 10.3%. The estimated mutated allele effect of FecLL and FecXGron litter size at +0.4 and +0.3 lamb per lambing in Noire du Velay and Mouton Vendéen, respectively. Due to the fairly high frequency and the rather strong effect of the FecLL and FecXGr prolific alleles, specific management programmes including genotyping should be implemented for a breeding objective of prolificacy adapted to each of these breeds.
Subjects
free text keywords: General Veterinary, Animal Science and Zoology, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, ovine, major gene, prolificacy, BMP15, B4GALNT2, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Autre (Sciences du Vivant), ovine;major gene;prolificacy;BMP15;B4GALNT2, Locus (genetics), Genetic gain, Allele, Genotyping, Breed, Biology, Domestic sheep reproduction, Veterinary medicine, Major gene, Population, education.field_of_study, education
32 references, page 1 of 3

Amer, P.R., Bodin, L., 2006. Quantitative genetic selection for twinning rate in ewes. N.Z. Soc. Anim. Product.

Bodin, L., Di Pasquale, E., Fabre, S., Bontoux, M., Monget, P., Persani, L., Mulsant, P., 2007. A novel mutation in the bone morphogenetic protein 15 gene causing defective protein secretion is associated with both increased ovulation rate and sterility in Lacaune sheep. Endocrinology 148, 393-400.

Bodin, L., Elsen, J.M., Station d'amelioration genetique des animaux, 1989. Variability of litter size of french sheep breeds following natural or induced ovulation. Anim. Product. 535-541.

Bodin, L., Raoul, J., Demars, J., Drouilhet, L., Mulsant, P., Sarry, J., Tabet, C., TosserKlopp, G., Fabre, S., Boscher, M.Y., 2011. Etat des lieux et gestion pratique des gènes d'ovulation détectés dans les races ovines françaises. 18èmes Rencontres Recherches Ruminants. Institut de l'Elevage, Paris, France, pp. 393-400.

Bradford, G.E., 1985. Selection for litter size. In: Land, R.B., Robinson, D.W. (Eds.), Genetics of Reproduction in Sheep. Butterworths, London, pp. 3-18.

Cottle, D.J., Gilmour, A.R., Pabiou, T., Amer, P.R., Fahey, A.G., 2016. Genetic selection for increased mean and reduced variance of twinning rate in Belclare ewes. J. Anim. Breed. Genet. 133, 126-137.

Davis, G.H., Montgomery, G.W., Allison, A.J., Kelly, R.W., Bray, A.R., 1982. Segregation of a major gene influencing fecundity in progeny of Booroola sheep. N.Z. J. Agric. Res. 25, 525-529.

Demars, J., Fabre, S., Sarry, J., Rossetti, R., Gilbert, H., Persani, L., Tosser-Klopp, G., Mulsant, P., Nowak, Z., Drobik, W., Martyniuk, E., Bodin, L., 2013. Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep. PLos Genet. 9, e1003482. [OpenAIRE]

Drouilhet, L., Lecerf, F., Bodin, L., Fabre, S., Mulsant, P., 2009. Fine mapping of the FecL locus influencing prolificacy in Lacaune sheep. Anim. Genet. 40, 804-812. [OpenAIRE]

Drouilhet, L., Mansanet, C., Sarry, J., Tabet, K., Bardou, P., Woloszyn, F., Lluch, J., Harichaux, G., Viguié, C., Monniaux, D., Bodin, L., Mulsant, P., Fabre, S., 2013. The highly prolific phenotype of Lacaune sheep is associated with an ectopic expression of the B4GALNT2 gene within the ovary. PLos Genet. 9, e1003809.

Fabre, S., Pierre, A., Mulsant, P., Bodin, L., Di Pasquale, E., Persani, L., Monget, P., Monniaux, D., 2006. Regulation of ovulation rate in mammals: contribution of sheep genetic models. Reproduct. Biol. Endocrinol. 4, 20. [OpenAIRE]

Fathallah, S., Alabart, J.L., Bodin, L., Jimenez-Hernando, M.A., Lahoz, B., Fantova, E., David, I., Jurado, J.J., 2016. Relaciones entre los efectos del gen BMP15 y los efectos poligénicos sobre la prolificidad en la raza ovina Rasa Aragonesa. Informacion Tecnica Economica Agraria 112, 45-56.

Galloway, S.M., McNatty, K.P., Cambridge, L.M., Laitinen, M.P.E., Juengel, J.L., Jokiranta, T.S., McLaren, R.J., Luiro, K., Dodds, K.G., Montgomery, G.W., Beattie, A.E., Davis, G.H., Ritvos, O., 2000. Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner. Nat. Genet. 25, 279-283. [OpenAIRE]

Gianola, D., 1982. Assortative mating and the genetic correlation. Theor. Appl. Genet. 62, 225-231.

Gilmour, A.R., Gogel, B.J., Cullis, B.R., Welham, S.J., Thompson, R., 2014. ASReml User Guide Release 4.1 Functional Specification. VSN International Ltd, Hemel Hempstead, United Kingdom. http://www.vsni.co.uk.

32 references, page 1 of 3
1 research outcomes, page 1 of 1
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