Enhanced Y1H assays for Arabidopsis
Copyright policy )Enhanced Y1H assays for Arabidopsis
We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.
- University of California, San Diego United States
- Agricultural Research Service United States
- University of California, Davis United States
- United States Department of Agriculture United States
- University of California, San Diego United States
580, 570, Systems Biology, Plant Sciences, Arabidopsis, Reproducibility of Results, Plant, Plant Roots, Promoter Regions, *Two-Hybrid System Techniques, Gene Expression Regulation, Genetic, Gene Expression Regulation, Plant, Two-Hybrid System Techniques, Promoter Regions, Genetic, Molecular Biology, Transcription Factors
580, 570, Systems Biology, Plant Sciences, Arabidopsis, Reproducibility of Results, Plant, Plant Roots, Promoter Regions, *Two-Hybrid System Techniques, Gene Expression Regulation, Genetic, Gene Expression Regulation, Plant, Two-Hybrid System Techniques, Promoter Regions, Genetic, Molecular Biology, Transcription Factors
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