Expression of Prolactin Gene in Mouse Placenta During Late Pregnancy: Detection of mRNA and Its Translation Product.
Copyright policy )Expression of Prolactin Gene in Mouse Placenta During Late Pregnancy: Detection of mRNA and Its Translation Product.
To examine the existence of PRL messenger ribonucleic acid (mRNA) in the mouse placenta during late pregnancy, reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blot analysis were carried out followed by nucleotide sequence analysis of cDNA. Total RNA extracted from each tissue was reverse-transcribed, followed by PCR with two oligonucleotide primers specific or a part of mouse PRL (mPRL) cDNA. An amplified RT-PCR product of predicted size was detected in all samples from the placenta of days 16 and 18 pregnant mice. This product was specifically hybridized with a probe overlapping an entire sequence of mPRL cDNA in Southern blot analysis. Nucleotide sequence analysis also provided evidence that the amplified cDNA had a nucleotide sequence completely identical to the mPRL cDNA sequence reported previously. Furthermore, mPRL with a slightly bigger molecular weight than that of pituitary PRL was detected in the placenta of days 12, 14, 16 and 18 pregnancy by immunoblot analysis. These results suggest that PRL mRNA and its translation product are synthesized in mouse placenta during late pregnancy.
- Meiji University Japan
- Meijo University Japan
Electrophoresis, Agar Gel, Male, Mice, Inbred ICR, Base Sequence, Placenta, Immunoblotting, Gene Expression Regulation, Developmental, Polymerase Chain Reaction, Prolactin, Blotting, Southern, Mice, Pregnancy, Animals, Electrophoresis, Polyacrylamide Gel, Female, RNA, Messenger, DNA Primers, Plasmids
Electrophoresis, Agar Gel, Male, Mice, Inbred ICR, Base Sequence, Placenta, Immunoblotting, Gene Expression Regulation, Developmental, Polymerase Chain Reaction, Prolactin, Blotting, Southern, Mice, Pregnancy, Animals, Electrophoresis, Polyacrylamide Gel, Female, RNA, Messenger, DNA Primers, Plasmids
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).15 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!