Expression of mRNA Isoforms of Latent Transforming Growth Factor-β Binding Protein-1 in Coronary Atherosclerosis and Human Tissues
Copyright policy )Expression of mRNA Isoforms of Latent Transforming Growth Factor-β Binding Protein-1 in Coronary Atherosclerosis and Human Tissues
Latent transforming growth factor-β binding protein-1 (LTBP1) has been implicated in the control of secretion, localization, and activation of TGFβ (transforming growth factor-β). We developed a quantitative reverse-transcriptase polymerase chain reaction (Q-RT-PCR) assay using an RNA internal standard to examine the expression of three alternatively spliced isoforms of LTBP1 (LTBP1Δ41, LTBP1Δ53, and LTBP1Δ55) in a variety of human tissues. The assays were also used to determine the expression of LTBP1L and LTBP1S isoforms and total LTBP1. The Q-RT-PCR assays were highly reproducible and showed that in most tissues LTBP1Δ55 and LTBP1L were minor components of LTBP1. The proportion of LTBP1Δ41 ranged from 2% of total LTBP1 mRNA in early coronary atherosclerotic lesions to 54% in advanced lesions.
- Harvard University United States
- Massachusetts General Hospital United States
- University of Cambridge United Kingdom
Reverse Transcriptase Polymerase Chain Reaction, Genetic Variation, Epithelial Cells, Coronary Artery Disease, Coronary Vessels, Alternative Splicing, Latent TGF-beta Binding Proteins, Organ Specificity, Case-Control Studies, Humans, Protein Isoforms, Female, RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction, Genetic Variation, Epithelial Cells, Coronary Artery Disease, Coronary Vessels, Alternative Splicing, Latent TGF-beta Binding Proteins, Organ Specificity, Case-Control Studies, Humans, Protein Isoforms, Female, RNA, Messenger
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